VONKOSSA

                                                            VONKOSSA

Principle

This method is based on the conversion calcium salt in the salt of another metal ( Silver ) which itself is opaque..

Solutions

A) Citrate buffer pH- 4.5
           0.2M Disodium hydrogen phosphate     -        283 gms
          Distilled Water                                              -        100ml

B ) 0.1M Citric acid
                   Citric acid              -        2.1 gm
                   Distilled wate -        100ml

Working solution
                              Solution A             -        9.09ml
                              Solution B             -        10.9
5%, Silver nitrate
           Silver nitrate          -        5 gms
           Distilled water        -        100ml .
1% Eosin.








Procedure
1) Deparaffinise two sections in xylene for 10mnts
2) Bring the section to water through descending series of alcohol .
3) Immerse one section in citrate buffer for 20mnts.
4) Wash both the slides well in distilled Water
5) Flood slides with silver nitrate.
6), Expose to bright sunlight or ultraviolet light for 30-45 minutes on to a 60 Watl electric bulb at a range of 4-5 inches for 30-60 minutes.
7) Wash in several changes of distilled water
8) Treat with 5% solution thiosulphate for 2-3 mnts
 9) Counterstain with eosin for 30 Secs
 10) Wash in water, dehydrate, clear and mount.


Result

            Calcium deposits           -           Black
             Background                  -           Pink


Comments

Post a Comment

Popular posts from this blog

PAS WITH AND WITHOUT DIASTASE

              PAS WITH AND WITHOUT DIASTASE Principle Section must be oxidized with periodic acid to convert 1:2 glycol group to          di-aldehyde in order that they will react with Schiff reagent to produce a colored end product. Glycogen is destroyed by the enzyme diastase which may be obtained from saliva or Malt Solutions Periodic Acid                 -           500mg Distilled Water               -           100 ml The solution is colourless All the glass wares should be properly cleaned and rinsed thoroughly in distilled Water. Procedure 1)    Add 1gm of basic fuchsin into 200 ml of hot distilled  water in a coni...
Read more

MELANIN BLEACH

                        MELANIN BLEACH Melanin is insoluble in almost all organic solvents. Strong oxidizing agent like potassium permanganate will bleach the melanin pigment. Solutions  1) 0.25% Potassium permanganate .                                     Potassium permanganate               -           250mg                                     Distilled water               ...
Read more

LUXOL FAST BLUE

                                      LUXOL FAST BLUE The  method is excellent for staining myelin fibres with good cellular differentiation. Solutions 1)     Luxol fast blue Solution . Luxol fast blue- 1 gm 95% alcohol-100 ml 10% acetic acid-5 ml 2)     Lithium Carbonate 0,05% Aqueous solution 3)     Haematoxylin .  Procedure 1)     Deparaffinise the section in xylene for 10 mts. 2)     Bring the section to water through descending series of alcohol. 3)     Stain in Luxol fast blue overnight at 37°C. 4)       Wash in 95% alcohol then in distilled water 5)     Commence differentiation by immersing sections in lithium carbonate solution for not more than 20 seconds 6)       Differen...
Read more