LUXOL FAST BLUE

                                      LUXOL FAST BLUE

The  method is excellent for staining myelin fibres with good cellular differentiation.

Solutions

1)    Luxol fast blue Solution.

Luxol fast blue- 1 gm

95% alcohol-100 ml

10% acetic acid-5 ml

2)    Lithium Carbonate

0,05% Aqueous solution

3)    Haematoxylin.

 Procedure

1)    Deparaffinise the section in xylene for 10 mts.

2)    Bring the section to water through descending series of alcohol.

3)    Stain in Luxol fast blue overnight at 37°C.

4)      Wash in 95% alcohol then in distilled water

5)    Commence differentiation by immersing sections in lithium carbonate solution for not more than 20 seconds

6)      Differentiate in 70% alcohol until grey and white matter are clearly distinguished (30 seconds to 1 minute).

7)      Wash well in distilled water.

8)    Counterstain with Haematoxylin for 1-2 mts

9)      Differentiate in 1% acid alcohol.

10)  Keep it in running tap water for 5-10 mts.

4)      1 l) Dehydrate, clear and mount.

Results

Myelin          -        Blue

Nuclei          -        Purple