VANGEISON STAIN

                        VANGEISON STAIN

Principle

The method depends upon thę of tissue and the size of the dye molecule . Thus collagen Whish is relatively permeable takes red colour with acid fuchsin and muscle which in far less permeable takes yellow with picric acid . Weigert’s haematoxylin which is mordanted to an iron salt avidity to withstand treatment  with picric acid . which effectively removes alum haematoxylin from nclei.

Solutions

1.       Weigert’s haematoxylin

Solution A

 Haematoxylin                       -           1gm

Absolute Alcohol                   -           100ml

Solution B

30% Aqueous ferric chloride           -           4ml

Concentrated Hydrochloric acid     -           1ml

Distilled water                                   -           95 ml

Mix equal quantities of A and B before use. The solution should be dark black in color .

2.      Vangeison solution ( Red Color )

  Saturated aqueous picric acid                   -           100ml

1% Acid Fuchsin       

Procedure

1.      Reparaffinise the section in Xylene for 5-10 mnts

2.      Bring the section it alè ugh descending series of alcohol

3.      Stain with Haematoxylin for 5-10 mts

4.      Wash it in water

5.       Differentiate it in 1% acid alcohol

6.      Keep it in running tap water for 5-10 mnts

7.      Stain with vangeison Solution for 2 mts.

8.      Drain the solution and blot with filter paper

9.      Picric acid will be dissolved when washed in water

10.  Clear and Mount.

Result

Collagen                      -           Red

Nuclei                          -           Blue

Muscle                                    -           Yellow