TOLUIDINE BLUE

                                                TOLUIDINE BLUE

Solution

Toluidine blue                -           1 ցոl

 Distilled water               -           100Ml

Procedure

1) Deparaffinise the section in Xylene for 10 mts

 2) Bring the section to Water through descending series of alcohol.

3) Stain with methyl violet for 3-5 mts

4) Section should be mounted in aqueous media.

5) Section is kept in water and examined in mediately under microscope.

Result

Amyloid is distinguished by its dark red polarization colour.