PERLS’S STAIN

                                PERLS’S   STAIN


Principle


Treatment with an acid ferrocyanide solution will result in the unmasking offerric iron in the form of hydroxide by dilute hydrochloric acid. The ferric iron then reacts with a dilute potassium ferrocyanide to produce an insoluble blue compound. ferric ferrocyanide (Prussian blue)


Solutions
1)          A) 10% Potassium ferrocyanide.
                         Potassium ferrocyanide      -           10gms
                        Distilled water                        -           100ml
            B) 10% Hydrochloric acid
                                     Hydrochloric acid     -           10ml
                                    Distilled water            -           90ml

Working solution
Equal parts of solution A and B. Glasswares should be thoroughly rinsed in
distilled water.

2)                Eosin 1%
                             Eosin                             -        1 gm.
                             Distilled water     -        100ml
          Few crystals of thymolis added as preservative.







Procedure

1)   Deparaffinise the section in Xylene for 10mts.
2)   Bring the section to water through descending series of alcohol.
3)   Rinse the section with distilled water.
4)   Treat the section with the working solution for 20minutes.
5)      Rinse in distilled water.
6)   Counterstain with eosin for 1 min.
7)      Wash it in water, dehydrate, clear and mount.



Result

 Iron pigments                    -       Blue
 Background                      -       Pink


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