MUCCARMINE

                                                      MUCCARMINE

Principle

Mucicarmine has got specific size. The aluminium salts in the solution form a chelate compound with carmine, thus conferring a net positive charge on the molecule and consequent binding to the tissue polyanion.

Solutions

1)    Mucicarmine solution

 Carmine                                       -                      l gm

            Anhydrous aluminium chloride           -                       0.5gm

            50% alcohol                                        -                      100ml

Take 2 ml distilled water in a test tube, add carmine and aluminium chloride. I leat it under flame, until the solution becomes syrupy. Dissolve the solution by adding 50% alcohol little by little. Cool, filter and store it at 4°C.

2)      Weigerts Iron haematoxylin

Solution A

                                Heamatoxylin                        -           1 gm

                         Absolute alcohol                   -           100ml

            Solution B

                                    30% Aqueous ferric chloride            -           4ml

                                    Concentrated hydrochloric acid        -           1 ml

                                     Distilled water                                   -           95 ml  

                        Mix equal amount of A and B before use.

3)      Metanit Yellow:- 0.25%

                                Metanil Yellow                        -           0.25 gm

                        Distilled water                        -           100 ml

Procedure

1)    Deparaffinise the section in xylene for 10 mts

2)   Bring the section to water through descending series of alcohol.

3)   Stain with iron Haematoxylin for 5-10 mts.

4)   Wash it in water. Differentiate in 1% acid alcohol.

5)   Keep it in running tap water for 5-10 mts

6)   Stain with mucicarmine solution for 45-60 mts. . (Dilute 1 part of stock solution with 3 parts of tap water).

7)    Wash it in distilled water. Counter stain with metanil yellow for 30 sec.

8)    Wash it in water, dehydrate, clear and mount.

 Result

                                    Mucin                               -       Pink

                                    Nuclei                               -      Black

                              Background                       -      Yellow.