FONTANA MASSON

                                      FONTANA MASSON



Principle

Melanin have the ability to reduce solution of ammoniacal silver nitrate to metallic silver.

Solutions

1)   Ammoniacal silver solution:
To 10% aqueous silver nitrate add strong ammonia. A precipitate will be formed.  Add ammonia drop by drop until the precipitate dissolves. Add 10% silver nitrate drop by drop, until a muddy color is formed. Dilute with equal amount of distilled water. Keep it in dark overnight.
2)     1% Sodium thiosulphate.
 Sodium thiosulphate             -        1gm
Distilled water                         -        100ml
3)     1% Eosin.
                   Eosin                             -        1 gm
                   Distilled water     -        100ml













Procedure


1) Deparaffinise the section in Xylene for 10 mts.
2) Bring the section to water through descending series Of alcohol.
 3) Rinse the section thoroughly in distilled water.
4) Keep the section in the beaker containing the ammoniacal silver solution at 60°C for 45-60 mts (Until light brown colour develops).
5) Wash it in distilled water.
 6) Treat with sodium thiosulphate for 2-3 mts.
7) Wash it in water.
8) Counterstain with eosin for 1 mt
9) Wash it in water, dehydrate, clear and mount
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Result

Argentaffin cells                         -                 Black
Background                              -                 Pink.


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