GOMORIS METHANAMINE SILVER

            GOMORIS METHANAMINE SILVER
Principle

The carbohydrates in the fungi are converted to dialdehyde by chromic acid treatment, which impregnate the silver.

Solutions

1)   Gomoris silver methanamine solution

                        5%, Silver nitrate                               -           5 ml
                        3% Hexamine in distilled water       -           100ml

Add silver nitrate to methanamine solution shaking until the precipitate which first forms dissolves. Store it in brown bottle at 4°C.

Working solution

 Silver methanamine solution                -        25 ml
Distilled water                                      -        25 ml
5% Borax                                           -        3ml

Glasswares should be washed well and rinsed thoroughly in distilled water. Metal forceps should not come in contact with the solution.

2) Gold chloride
                        Gold chloride                         -           200 mg
                        Distilled water                        –          100 ml

3)    3% sodium thiosulphate
                             Sodium thiosulphate                   -       3 gm
                              Distilled water                           -        l00 ml

4)    Light green
                   light green                     -        200 ml
                   0.2% acetic acid           -        100ml
Procedure
1.   Deparaffinise in Xylene for 10 mts.
2.    Bring the section to water through descending series of alcohol.
3.   Oxidise with chromic acid for 30 mts
4.   Rinse the section thoroughly in distilled water.
5.    Keep the section in a beaker containing the working solution, inside the incubator at 60°C.
6.    Check for the development of light brown colour, usually it takes around 60 minutes for the development of brown colour.
7.   Wash it in distilled water.
8.    Treat with gold chloride for 10 mts wash it in water.
9.    Treat with sodium thiosulphate for 2-3 mts
10.  Wash it in water.
11. Counter stain with light green for 30 sec. - 1 minute.
12. Wash it in water, dehydrate, clear and mount.


Result

PAS positive structures, basement membrane, fungi, mucin etc. - Black to Brown

Background - Green.

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