FAULTS INSECTION CUTTING

                             FAULTS INSECTION CUTTING

        FAULTS   

  REASON AND REMEDY

Sections cut vertically (SCORES)
                                                                                                                         -Knife contains small nicks
 -Knife edge to be cleaned
-Tissue has calcified areas
Sections cut or roll up
-Knife is blunt
- Tilt of knife is too-much ( so reduce clearance angle)
Sections are alternatively thick and thin
Microtome adjusting Screws need-tightening
Sections crumble on cutting
-Knife is blunt
- Wax is too soft and needs ice applied to the Cutting-Surface
- Wax is crystallized due to slow cooling or contamination with water or  clearing agent
 Ribbon of sections curved
-Block-edges-are not-parallel-to-each other
- Block edges are not parallel to knife edge



Serial deeper - Take ribbon sections, trim a little bit and again take ribbon sections. Like this take 3-4 ribbon sections.

Deeper -  If the whole section is not exposed, adjust the position of the block, so that the unexposed portion is trimmed first. Trim until the whole the section is exposed and take ribbon sections. If the whole section is exposed trim a little bit and take ribbon section.

Thinner-  Reduce the micron adjustment to 1-2µ  and cut slowly and take section as thin as possible.




Rotate block
Some tissues need orientation e.g.-tubes should embed perpendicularly. If it is embedded longitudinally melt the block, rotate the tube by 90* and reembed it. ' Sometimes, the cutting surface will be the reverse side. Melt the tissue rotate it 80° and reembed.


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