CONGO RED

                                                CONGO RED


Principle
The staining is based on the selective affinity of the dye Congo red for amyloid.

Solutions
1)     1% Congo red (orange colour)
 Congo red                 -           1 gm
 Distilled water           -           100 ml

 2) Haematoxylin,
3) Saturated Lithium Carbonate.
Procedure

1) Deparaffinise the section in xylene for 10mts.
2) Bring the section to water through descending series of alcohol.
 3) Stain with Congo red for 30 mts.
 4) Wash with tap water
.5) Flood slide with saturated aqueous solution of lithium carbonate for 15 seconds
6) Keep it in running tan water for 5-10 mt
7) Counterstain with Haematoxylin for 1-2 mts
8) Wash it in water
9) Differentiate it in 1% acid alcohol.
 10) Keep it in running tap water for 5-10 mts
11) Dehydrate, clear and mount


Result -

            Amyloid           -                    Pink to red.
              Nuclei               -                      Blue


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