ALZARIN RED

                                ALZARIN RED


Principle


This method is based on the ability of certain dyes (alizarin red) to form lakes with calcium salts.

Solutions

                                    Alizarin red                  -           1gms
                                    Distilled water             -           50ml

Add 0.5% ammonia until pH 4.2 is reached, using a pH meter. The solution should be deep iodine in colour.

Procedure

1) Deparaffinise the section in xylene for 10mts.
2) Bring the section to water through descending series of alcohol.
 3) Place the section in a coplin jar filled with alizarin solution for 1-5 minutes.
 4) Blot and rinse in acetone for 30 seconds.
5) Treat with equal parts of acetone Xylene for 15 seconds.
 6) Rinse in Xylene and mount.


Results

                        Calcium deposits         -        Orange red
                   Background                  -        Pink


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