ALCIAN BLUE — PAS

                        ALCIAN BLUE — PAS

Principle
Alcian blue Solutions of varying PH are used to separate and identify the different acid
mucins.


Solutions
 1) Alcian blue - 1 gm

PH-02
10% Sulphuric acid     -       100ml

PH-0.5
 0.2 M Hydrochloric acid      -     100ml

PH-10
0.1 M Hydrochloric Acid          -       10Oml

PH-2.5
3% Acetic acid                        -        100ml

PH-3.2
0.5%o Acetic acid    -           100ml.

2).5% Periodic acid
 Schiff reagent
Haematoxylin






Procedure.
1)    Deparaffinise the section in xylene for 10mnts
2)      Bring the section to water through descending series of alcohol
3)       Treat with Alcian blue solution for 20mts.
4)       Wash it in running tap water,
5)       Add 0.5% periodic acid for 10mts.
6)      Wash it in water and rinse thoroughly in distilled water
7)      Add Schiff reagent for 20mts.
8)      Кеер it in running tap water for 5-10 mnts.
9)      Counter stain with Haematoxylin for 1-2ms.
10)  Wash it in Water.
11)  Differentiate in 1% acid alcohol.
12)  Keep it in running tap water for 5-10mts.
13)   Dehydrate, clear and mount.



Result.

Acid mucin                       -          Blue
Neutral mucin                   -           Pink
Nuclei                               -           Blue.
14)  21
15)  6) Wash it in Water and rinse thoroughly in distilled water.
16)    


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